Table 2.
Effect of pyruvate on LDH enzyme kinetics in cell extracts
| LDH enzymes | Vmaz (ΔAbs/s∗10−3/μg protein) | Vpeak (ΔAbs/s∗10−3/μg protein) | Cmax (mM) | Km (mM) | Ki (mM) |
|---|---|---|---|---|---|
| Panc03.27 | 2.00 ± 0.07 | 1.56 | 0.33 | 0.05 ± 0.00 | 2.33 ± 0.21 |
| Mia Paca-2 | 0.88 ± 0.03 | 0.66 | 0.69 | 0.11 ± 0.01 | 4.26 ± 0.32 |
| QGP1 | 1.85 ± 0.05 | 1.42 | 0.51 | 0.08 ± 0.01 | 3.42 ± 0.25 |
| MDA-MB-231 | 2.98 ± 0.17 | 2.20 | 0.60 | 0.10 ± 0.02 | 3.39 ± 0.50 |
| HPNE | 2.71 ± 0.08 | 1.98 | 0.48 | 0.09 ± 0.01 | 2.67 ± 0.20 |
Abbreviation: LDH, lactate dehydrogenase.
Summary of maximal initial velocities from activation curve fits (Vmax), peak initial velocities of the biphasic curve fits (Vpeak), pyruvate concentrations at peak velocities of the biphasic curve fits (Cmax), and apparent Km and Ki values of LDH in extracts from Panc03.27, Mia Paca2, QGP1, MDA-MB-231, and HPNE cells. All experiments were done in triplicate (technical replicates); n = 3, independent experiments (biological replicates); mean values with standard errors.