Skip to main content
. 2021 May 26;297(1):100830. doi: 10.1016/j.jbc.2021.100830

Figure 1.

Figure 1

Identifying dietary regulation of genes in neurons and astrocytes using translating ribosomal affinity purification (TRAP).A, graphic showing possible metabolic outcomes of phosphoethanolamine (PEtN). B, Etnppl mRNA expression using qRT-PCR of input and pulled-down (IP) neuron (Syn-Cre) and astrocyte (Aldh1l1-Cre) fractions tissue harvested from ribo-tag mice exposed to fed, fasted diet, or ketogenic diet of TRAP screen. Samples sizes [Syn-Cre: fed (n = 5), fasted (n = 7), ketogenic diet (n = 6)]. Aldh1l1-Cre: fed (n = 5), fasted (n = 4), ketogenic diet (n = 8). C, protein expression of Etnppl from fed and overnight fasted adult mice n = 3. D, Etnppl mRNA expression using qRT-PCR across ages (prenatal day 18 (E18) and postnatal day 7 and 35 (P7 and P35) and CNS regions (cortex, spine, and hippocampus)). (n = 3) Data are expressed as mean ± S.D. Represented data analyzed using multiple Student’s two-tailed t-tests. ∗α = 0.05; ∗∗α = 0.01; ∗∗∗α = 0.001; ∗∗∗∗α = 0.0001; ns, not significant.