Expression of Etnppl and PEtN-related genes across early development.A, Etnppl protein expression in the whole brain across ages. WT and EtnpplKO fasted cerebellum (CB) tissue used as positive and negative controls for Etnppl protein expression repectively. B, Etnppl protein expression in the liver across ages. WT and EtnpplKO fasted CB and liver tissue used as positive and negative controls for Etnppl protein expression repectively. C, Etnppl and PEtN-related gene mRNA expression in the whole brain using qRT-PCR across ages P3 (n = 3), P4 (n = 4), P14 (n = 3), P21 (n = 4), P28 (n = 4). D, Etnppl and PEtN-related gene mRNA expression in the liver using qRT-PCR across ages P3 (n = 3), P4 (n = 4), P14 (n = 3), P21 (n = 4), P28 (n = 4). Data (as mentioned in text) are expressed as mean ± S.D. Represented data analyzed using ordinary measures two-way analysis of variance with Sidak’s tests for multiple comparisons. Outliers were removed after using Grubb’s outlier test. ∗α = 0.05; ∗∗α = 0.01; ∗∗∗α = 0.001; ∗∗∗∗α = 0.0001; ns, not significant.