Examining regulation of PEtN-related genes by diet and glucocorticoids.A, mRNA expression of Etnppl and other PEtN-related genes in the whole brain from adult chow-diet-fed, fasted, and refed after fasted mice using qRT-PCR. (n = 8). B, mRNA expression of Etnppl and other PEtN-related genes in the liver from adult chow-diet-fed, fasted, and refed after fasted mice using qRT-PCR. (n = 8). C, mRNA expression of PEtN-related genes in wild-type P2 1° astrocytes after a 24- or 72-h exposure to the glucocorticoid agonist dexamethasone. [dexamethasone] = 100 nM. (n = 3). Data in A and B are expressed as mean ± S.E.M. Represented data analyzed using ordinary measures two-way analysis of variance with Sidak’s tests for multiple comparisons. Data in C is expressed as mean ± S.D. Represented data analyzed using Student’s two-tailed t-tests. ∗α = 0.05; ∗∗α = 0.01; ∗∗∗α = 0.001; ∗∗∗∗α = 0.0001; ns, not significant.