Figure 5.

Loss of Etnppl does not result in major changes to oxygen consumption or abundance of many hippocampal PEtN-related metabolites.A, seahorse assay mitochondrial stress test measuring oxygen consumption using cultured P2 1° cortical astrocytes derived from Etnppl^KO and WT mice after overnight incubation with dexamethasone and ethanolamine (EtN) (n = 6). [dexamethasone] = 100 nM, [EtN] = 5 mM. B, relative abundances of PEtN-associated metabolites in whole hippocampus from 18-h fasted 9-week-old Etnppl^KO and WT (n = 6). Data in A are expressed as mean ± S.E.M. Represented data analyzed using multiple Student’s two-tailed t-tests. Statistical significance of represented metabolites in B determined using two-stage false discovery rate (FDR) method of Benjamini, Krieger, and Yekutieli with an FDR (Q) of 10%. Fold changes in green boxes are significantly increased, fold changes in red boxes are significantly decreased, and fold changes in yellow boxes are not significantly affected by genotype. The same data are represented as mean of relative species abundance ± S.D. in adjacent graphs in panels C–E. ∗α = 0.05; ∗∗α = 0.01; ∗∗∗α = 0.001; ∗∗∗∗α = 0.0001; ns, not significant.