Fig. 1.
LSFM-based mouse brain atlas. a) Generation of a brain template based on the LSFM autofluorescence volumes of 139 mice brains using an iterative registration and averaging algorithm. Raw light sheet scans are annotated with Ix where x stands for the animal number, and the intermediate average mouse brain volumes are annotated with Ay where y stands for the iteration step. B) Transfer of brain region segmentations from the AIBS CCFv3 to the LSFM mouse brain template. Brain regions of the AIBS CCFv3 were mapped to the LSFM template in six parts, e.g. cortex to cortex, hindbrain to hindbrain etc.