Table 1.

Summary of viability assay and HER2 IHC staining results

Cell line	Viability assay relative IC50 (µg/mL)		Fold change	HER2 IHC intensity level
	2D	3D
BT-474	0.178	6.71	37.7	3+
SK-BR-3	0.00876	0.0366	4.18	3+
MDA -MB-361	0.0912	1.99	21.8	2+
MDA-MB-175 VII	0.35	1.49	4.3	1+
MCF-7	17.3	16.8	0.974	1+

The IC₅₀ results from Fig. 2 are shown for the five cell lines grown as 2D and 3D cultures. The calculated IC₅₀ fold change and HER2 IHC staining intensities are also shown