Fig. 7.
iRhom2-dependent ADAM17 activity in iRhom2W538S/W538S mice. BMDMs from mice homozygous for either iRhom2_W538S or for an iRhom knock out (Del) and BMDMs from their respective wild type littermates (wt) were treated for 24 h with 100 ng/ml LPS or with PBS as negative control. a LPS-stimulated release of IL6, CXCL1, TNFα and TNFRI were measured by ELISA. W538S: n = 5, Del: n = 3 b Induction of the IL6 and iRhom2 mRNA expression upon stimulation with LPS was measured by qPCR. n = 3. Additionally, upregulation of iRhom2 on protein level was determined. Expression of endogenous iRhom2 and ADAM17 in BMDMs was detected by precipitating glycosylated proteins with concanavalin A beads and immunoblotting. Transferrin receptor (TfR) served as input control. BMDMs were derived from mice homozygous for either iRhom2_W538S or wild-type (wt). n > 3. c TNFRI levels in serum from iRhom2W538S/W538S mice as well as from their wild type littermates were determined by ELISA. W538S: n = 5