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. 2021 May 5;78(11):5015–5040. doi: 10.1007/s00018-021-03845-3
Gene Sequence Annealing temperature
mAdam17

For AAA CCA GAA CAG ACC CAA CG

Rev GTA CGT CGA TGC AGA GCA AA

57 °C
mGapdh

For CAT GGC CTT CCG TGT TCC TA

Rev ACT TGG CAG GTT TCT CCA GG

60 °C
mIl6

For TGC AAG AGA CTT CCA TCC AGT TGC C

Rev AAG CCT CCG ACT TGT GAA GTG GT

59 °C
miRhom1

For TTC TTC ACT TAC TGG CTC AC

Rev TTC CGA AGT ACC GAG TCC

60 °C
miRhom2

For AGA GCG TGA AGT ACA TCC

Rev TAA AGT CTC CGA GCA GTC C

60 °C
mRps29

For CCT TTC TCC TCG TTG GGC

Rev GAG CAG ACG CGG CAA

61 °C