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. 2021 Jun 25;4:799. doi: 10.1038/s42003-021-02215-w

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Midostaurin (Mid) IC50/cell viability and FLT3 glycosylation/phosphorylation analysis in MV4-11/MOLM-13 Mid sensitive (MID-Sens) and resistant (MID-Res) cells. b FLT3 phosphorylation/glycosylation was analyzed in MV4-11/MOLM-13 MID-Sens ± MID and MID Res ± MID by Western blot. FLT3 blot, G means glycosylated band (160 kDa) and NG, non glycosylated band (130 kDa). c FLT3 surface expression was analyzed in MV4-11/MOLM-13 MID-Sens ± MID and MID Res ± MID by flow cytometry. d FLT3 receptor was analyzed in MV4-11 MID-Res versus MID-Sens by super-resolution microscopy dSTORM. e RAC1 activation was analyzed in MV4-11/MOLM-13 MID-Res ± Mid 50 nM compared to MID-Sens cells ± Mid 50 nM (MID-Sens + Mid 50 nM versus MID-Sens − Mid (MV) p = 0.059, t = 3.4, df = 2.3 (MO) p = 0.07, t = 3.1, df = 2.3; MID-Res − Mid versus MID-Sens − Mid (MV) p < 0.01, t = 6.1, df = 3.8 (MO) p < 0.01, t = 5.7, df = 3.9; MID-Res + Mid versus MID-Sens (MV) p < 0.01, t = 10.03, df = 2.7 (MO) p < 0.01, t = 6.5, df = 3.4). f RAC1 activation was analyzed in FLT3 KD MV4-11/MOLM-13 MID-Res compared to scramble MV4-11 MID-Res cells ((MV) p < 0.01, t = 8.56, df = 2.9, (MO) p < 0.01, t = 5.1, df = 3.5). Relative light units (RLU). The western blot results are normalized by loading control (GAPDH) and are expressed as fold change relative to the control. Data are shown as means ± SDs (error bars) from three independent experiments.