Table 3.
Validation of the abundant miRNAs in the blood of women presenting at an infertility clinic as determined by RT-qPCR (P < 0.05). LAMH (n = 12) compared to NAMH group (n = 13), HAMH (n = 13) compared to NAMH group (n = 13), LAMH (n = 27) compared to NAMH group (n = 67), and HAMH (n = 27) compared to NAMH group (n = 67).
| LAMH (n = 12) compared to NAMH group (n = 13) | LAMH (n = 27) compared to NAMH group (n = 67) | ||||||
|---|---|---|---|---|---|---|---|
| Phase II—Validation of screening phase by RT-qPCR | Phase III—Independent validation by RT-qPCR | ||||||
| MicroRNA | P value | Log2 FC | Regulation | MicroRNA | P value | Log2 FC | Regulation |
| miR-144-5p | 0.0297 | − 1.90 | Lower | miR-144-5p | 0.0102 | − 1.66 | Lower |
| miR-7-1-3p | 0.0138 | − 1.62 | Lower | miR-7-1-3p | 0.0365 | − 0.71 | Lower |
| miR-126-3p | 0.0083 | − 1.51 | Lower | miR-126-3p | 0.0112 | − 1.65 | Lower |
| miR-146b-5p | 0.0025 | − 1.26 | Lower | miR-146b-5p | 0.0282 | − 0.71 | Lower |
| miR-338-3p | 0.0116 | − 1.41 | Lower | miR-338-3p | 0.0064 | − 1.11 | Lower |
| miR-3200-3p | 0.0225 | − 3.02 | Lower | miR-3200-3p | 0.0264 | − 2.09 | Lower |
| miR-330-3p | 0.0206 | − 1.55 | Lower | miR-330-3p | 0.0309 | − 1.33 | Lower |
| miR-21-5p | 0.0076 | − 1.53 | Lower | miR-21-5p | 0.0111 | − 1.45 | Lower |
| miR-7-5p | 0.0120 | − 2.20 | Lower | miR-7-5p | 0.0049 | − 1.47 | Lower |
| miR-660-5p | 0.0090 | − 1.21 | Lower | miR-660-5p | 0.0225 | − 0.85 | Lower |
| miR-148b-3p | 0.0011 | − 1.99 | Lower | ||||
| miR-199b-5p | 0.0355 | − 1.33 | Lower | miRNAs detected only in the independent sample seta | |||
| miR-502-5p | 0.0339 | − 1.36 | Lower | ||||
| miR-27a-3p | 0.0037 | − 1.05 | Lower | ||||
| miR-374b-5p | 0.0285 | − 1.01 | Lower | ||||
| miR-140-5p | 0.0144 | − 1.15 | Lower | ||||
| HAMH (n = 13) compared to NAMH group (n = 13) | HAMH (n = 27) compared to NAMH group (n = 67) | ||||||
|---|---|---|---|---|---|---|---|
| Phase II—Validation of Screening phase by RT-qPCR | Phase III—Independent validation by RT-qPCR | ||||||
| MicroRNA | P value | Log2 FC | Regulation | MicroRNA | P value | Log2 FC | Regulation |
| miR-3125 | 0.0139 | 1.74 | Higher | miR-100-5p | 0.0006 | − 2.67 | Lower |
| miR-483-5p | 0.0028 | 2.18 | Higher | miR-3200-3p | 0.0003 | − 3.25 | Lower |
| miR-100-5p | 0.0001 | − 3.87 | Lower | miR-7-1-3p | 0.0001 | − 1.45 | Lower |
| miR-3200-3p | 0.0274 | − 3.06 | Lower | miR-18a-5p | 0.0042 | − 2.00 | Lower |
| miR-7–1-3p | 0.0279 | − 1.49 | Lower | miR-17-3p | 0.0394 | − 1.44 | Lower |
| miR-18a-5p | 0.0146 | − 1.97 | Lower | miR-133b | 0.0091 | − 1.18 | Lower |
| miR-17-3p | 0.0226 | − 1.43 | Lower | miR-221-3p | 0.0046 | − 1.34 | Lower |
| miR-133b | 0.0281 | − 1.70 | Lower | miR-18b-5p | 0.0120 | − 1.78 | Lower |
| miR-654-3p | 0.0412 | − 1.27 | Lower | miR-744-5p | 0.0076 | − 0.65 | Lower |
| miR-221-3p | 0.0194 | − 1.68 | Lower | miR-10a-5p | 0.0310 | − 0.78 | Lower |
| miR-18b-5p | 0.0063 | − 2.02 | Lower | miR-28-5p | 0.0001 | − 1.37 | Lower |
| miR-744-5p | 0.0201 | − 0.60 | Lower | miR-330-3p | 0.0008 | − 1.69 | Lower |
| miR-10a-5p | 0.0062 | − 1.52 | Lower | miR-660-5p | 0.0115 | − 1.02 | Lower |
| miR-28-5p | 0.0059 | − 1.36 | Lower | miR-183-5p | 0.0019 | − 1.41 | Lower |
| miR-181a-2-3p | 0.0003 | − 1.92 | Lower | miR-148b-3p | 0.0008 | − 1.53 | Lower |
| miR-330-3p | 0.0107 | − 1.87 | Lower | ||||
| miR-660-5p | 0.0380 | − 0.93 | Lower | miRNAs detected only in the independent sample seta | |||
| miR-183-5p | 0.0155 | − 1.47 | Lower | miR-27a-3p | 0.0001 | − 1.70 | Lower |
| miR-199b-5p | 0.0050 | − 2.93 | Lower | miR-148a-3p | 0.0090 | − 1.49 | Lower |
| miR-505-3p | 0.0114 | − 1.69 | Lower | miR-7-5p | 0.0035 | − 1.57 | Lower |
| miR-148b-3p | 0.0125 | − 1.39 | Lower | miR-96-5p | 0.0151 | − 0.91 | Lower |
| miR-199a-5p | 0.0349 | − 1.52 | Lower | miR-500b-5p | 0.0000 | − 1.20 | Lower |
| miR-23b-3p | 0.0425 | − 1.06 | Lower | miR-144-5p | 0.0108 | − 1.80 | Lower |
| miR-140-5p | 0.0270 | − 1.11 | Lower | ||||
| miR-338-3p | 0.0002 | − 1.55 | Lower | ||||
| miR-182-5p | 0.0002 | − 3.16 | Lower | ||||
Unpaired t-test was used to calculate the P value.
Benjamini–Hochberg correction for multiple testing was used to adjust the P value.
P values of < 0.05 was considered statistically significant.
Relative abundance level of 2_ΔΔCt was used for RT-qPCR.
LAMH low anti-Müllerian hormone, HAMH high anti-Müllerian hormone, NAMH normal anti-Müllerian hormone, FC fold change.
aAdditional miRNAs were only detected by RT-qPCR in the independent validation phase and were not previously detected by microarray in the screening phase.