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. 2021 Jun 25;12:3972. doi: 10.1038/s41467-021-24211-8

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Left: schematic of cranial window placement and whiskers for stimulation. Right: enlarged view showing locations of the stroke (C1, magenta) and spared barrel (D3, green) highlighted. b Experimental timeline: ISI and 2P imaging were carried out at baseline (before stroke) and +5 days, +13 days, and +1 month after stroke. c Cresyl violet-stained coronal section from a representative mouse 5 days post-stroke. Scale bar = 1 mm. Inset: higher magnification view of infarct core, outlined in yellow. Scale bar = 250 μm. d ISI maps elicited by stimulation of C1 (magenta) and D3 (green) whiskers overlaid on photographs of the cranial window in representative sham control (top) and stroke (bottom) mice before and after stroke. Pale areas (yellow outline) represent the infarct. Note that the infarct size appears smaller over time as a result of the disappearance of acute tissue edema and tissue involution/scarring. Scale bar = 0.5 mm. e Quantification of C1 (solid lines) and D3 (dashed lines) whisker-evoked ISI map area size throughout recovery in control (gray) vs. stroke (red) animals (n = 4 and 6, respectively). Two-way repeated measures ANOVA, main effects of timepoint (T, #p = 0.042) and group-by-timepoint interaction (G:T, *p = 0.014). Significance for multiple comparison testing using the Tukey-Kramer procedure of timepoints (compared to baseline; #p < 0.05; ##p < 0.01); and group-by-timepoint interactions (comparing C1 and D3 map area size in the stroke group; *p < 0.05; **p < 0.01; ***p < 0.01). Data in e and f represent mean ± s.e.m. f Quantification of C1 (solid lines) and D3 (dashed lines) whisker-evoked ISI mean signal intensity over background signal throughout recovery in control (gray) vs. stroke (red) animals (n = 4 and 6, respectively). Two-way repeated measures ANOVA, main effects of timepoint (T, ##p = 0.003) and group-by-timepoint interaction (G:T, *p = 0.03). Significance for multiple comparison testing using the Tukey-Kramer procedure of timepoints (compared to baseline; #p < 0.05; ##p < 0.01;) and group-by-timepoint interactions (comparing C1 and D3 signal intensity in the stroke group; *p < 0.05; **p < 0.01).