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. 2021 Jun 25;11:13338. doi: 10.1038/s41598-021-92758-z

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Loss of rgs4 function alters mTOR signaling in neural cells (A) Immunoblotting of lysates from zebrafish embryos at 20 hpf. S6 and p-S6 amounts were normalized to α-tubulin and the ratio of p-S6 relative to S6 was compared between WT and MZrgs4 lysates. No significant difference was observed between the two groups. Immunoblotting of lysates from zebrafish embryos at 48 hpf. S6 and p-S6 amounts were normalized to β-actin and the ratio of p-S6 relative to S6 was compared between WT and MZrgs4 lysates. MZrgs4 embryos show a significant decrease in the amount of p-S6/S6 in comparison to WT. (B) Immunoblotting of neural lysates from zebrafish embryos at 48 hpf. S6 and p-S6 amounts were normalized to β-actin and the ratio of p-S6 relative to S6 was compared between WT and MZrgs4 lysates. MZrgs4 embryos showed a significant decrease in the amount of p-S6/S6 in comparison to WT.