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. 2021 Jun 25;12:3968. doi: 10.1038/s41467-021-24243-0

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a The number of FIREs detected in NeuN+ and NeuN− cells. b Differential FIREs were identified in NeuN+ and NeuN− cells. c, d Differential FIREs overlap with differential H3K27ac peaks in the corresponding cell types. A neuronal gene, GRIN2B, is located in NeuN+ specific FIREs (e), while two oligodendrocytic genes, OLIG1 and OLIG2, are located in NeuN− specific FIREs (f). FIREs and significance of FIRE scores in NeuN+ and NeuN− cells are depicted in green and purple, respectively. Boxplots in the right show expression levels of GRIN2B (FDR = 3.71e−12), OLIG1 (FDR = 7.34e−26) and OLIG2 (FDR = 8.06e−23) in NeuN+ (n = 4) and NeuN− (n = 4) cells. FPKM Fragments Per Kilobase of transcript per Million mapped reads. Center, median; box = 1st to 3rd quartiles (Q); minima, Q1 − 1.5 × interquartile range (IQR); maxima, Q3 + 1.5 × IQR. *FDR < 0.05 calculated by DESeq2 (two-sided Wald test). Source data are provided as a Source Data file. Gene ontology (GO) analysis for genes assigned to differential NeuN+ (g) and NeuN− (h) FIREs. The red line denotes FDR = 0.05. i Cellular expression levels of genes assigned to differential NeuN+ and NeuN− FIREs. Center, median; box = Q1−Q3; minima, Q1 − 1.5 × IQR; maxima, Q3 + 1.5 × IQR. Neurons, n = 131; astrocytes (Astro), n = 62; microglia (Micro), n = 16; endothelial (Endo), n = 20; oligodendrocytes (Oligo), n = 38. Source data are provided as a Source Data file. j Genes assigned to differential NeuN+ and NeuN− FIREs are enriched in neurons and glia, respectively. Ex excitatory neurons, In inhibitory neurons.