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. 2021 Jun 25;11:13317. doi: 10.1038/s41598-021-91747-6

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Flow cytometry analysis of DNA content of T. brucei cells treated with selected ANPs 24a, 24b, 28 and 27 at the concentration ranging from 1 to 16 µM depending on the tested inhibitor. Histograms show the distribution of DNA content of trypanosomes after exposure to selected ANPs. (A) Fluorescence intensity in the PE-A channel is shown on the x axis and number of cells on the y axis (example for 24b); (B) Bar chart displaying the percentage of cell at G0/G1, S and G2 phase. (means ± S.D., n = 3–7, Student’s unpaired test, ***p < 0.001, **p < 0.01, *p < 0.05).