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. 2021 Jun 25;12(7):650. doi: 10.1038/s41419-021-03935-x

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — A Transwell and martrigel assay of osteosarcoma cells with CT45A1 overexpression. B Transwell and martrigel assay of osteosarcoma cells with CT45A1 silencing. C Western blotting for the quantification of EMT markers in osteosarcoma cell lines with CT45A1 overexpression. D Western blotting of EMT markers in osteosarcoma cell lines with CT45A1 silencing. Scale bars: 100 μm (A, B). **P < 0.01, compared to the control. The error bars indicate the means ± SDs.