Table 2.

MCT4 expression in NSCLC tissues with or without predicted pathogenic mutations.

Patient ID	Histology	P/M	ND gene	Mutation	Amino acid change	Evolutionary conservation	MutPred score	Disease association	Homo/ Heterogen/ Hetero	IHC score
LuAdBrM5	Adenocarcinoma	M	ND1	T3394C	Y30H	Conserved	0.783	LHON/Diabetes/ CPT deficiency/ High altitude adaptation	Homo	+  +  +  +
LuAdBrM8	Adenocarcinoma	M	ND1	T3394C	Y30H	Conserved	0.783	LHON/Diabetes/ CPT deficiency/ High altitude adaptation	Homo	+  +  +
LuAdBrM13	Adenocarcinoma	M	ND1	T3394C	Y30H	Conserved	0.783	LHON/Diabetes/ CPT deficiency/ High altitude adaptation	Homo	+  +  +
LgCa196	Adenocarcinoma	P	ND1	C3497T	A64V		0.413	LHON	Homo	+  +  +
LuAdBrM2	Adenocarcinoma	M	ND1	C3497T	A64V		0.413	LHON	Homo	+  +  +  +
LuPoBrM1	Adenocarcinoma	M	ND1	C3497T	A64V		0.413	LHON	Homo	+  +  +
LgCa169	Squamous cell carcinoma	P	ND1	H3709A	A135T	Conserved	0.774		Heterogen or Hetero	− and +  +  +  +
LuAdBrM1	Adenocarcinoma	M	ND5	C12813A	Y159stop				Heterogen or Hetero	+  +  +  +
LgCa173	Squamous cell carcinoma	P	ND5	G13103A	G256E	Conserved	0.834		Heterogen or Hetero	+  +
LgCa157	Squamous cell carcinoma	P		None						+  +  +
LgCa179	Adenocarcinoma	P		None						+  + (local)
LgCa183	Squamous cell carcinoma	P		None						+
LgCa188	Squamous cell carcinoma	P		None						+
LgCa193	Squamous cell carcinoma	P		None						+
LgCa202	Adenocarcinoma	P		None						+
LgCa175	Adenocarcinoma	P		None						+
LgCa195	Bronchioalveolar carcinoma	P		None						+

Homo/Heterogen/Hetero: Homoplasmy/Heterogeneous/Heteroplasmy. Heterogeneous means a mixture of cancer cells with the mutation, those without the mutation and/or host-derived stromal cells with wild-type mtDNA.

P/M, Primary tumour/Metastases.