Table 3.
Inhibition of crown gall tumors by the plant extracts.
| Test material | Dose (μg/mL) | Tumor count per disc* | Inhibition of tumor [%] | IC50 (μg/mL) |
|---|---|---|---|---|
| Negative control | – | 37.33 ± 1.05 | ||
| Vincristine C | 1000 | 0.00 ± 0.00 | 100.00 | 9.8 |
| 500 | 0.00 ± 0.00 | 100.00 | ||
| 250 | 1.79 ± 0.59 | 95.20 | ||
| 125 | 2.13 ± 0.93 | 94.29 | ||
| 62.5 | 3.41 ± 0.72 | 90.87 | ||
| 31.25 | 3.93 ± 0.49 | 89.47 | ||
| 15.625 | 4.40 ± 1.21 | 88.21 | ||
| Etoposide | 1000 | 0.00 ± 0.00 | 100.00 | 9.00 |
| 500 | 0.00 ± 0.00 | 100.00 | ||
| 250 | 1.93 ± 0.21 | 94.83 | ||
| 125 | 2.85 ± 0.84 | 92.37 | ||
| 62.5 | 3.39 ± 0.30 | 90.92 | ||
| 31.25 | 4.08 ± 0.30 | 89.07 | ||
| 15.625 | 4.25 ± 1.17 | 88.62 | ||
| M.Az/F | 1000 | 4.63 ± 1.15 | 87.60 | 138.517 |
| 500 | 9.83 ± 0.92 | 73.67 | ||
| 250 | 17.89 ± 2.06 | 52.08 | ||
| 125 | 21.95 ± 2.71 | 41.20 | ||
| 62.5 | 24.78 ± 3.61 | 33.62 | ||
| 31.25 | 26.93 ± 0.94 | 27.86 | ||
| 15.625 | 28.75 ± 1.79 | 22.98 | ||
| M.Az/L | 1000 | 12.84 ± 1.40 | 65.60 | 325.596 |
| 500 | 17.04 ± 1.39 | 54.35 | ||
| 250 | 20.48 ± 2.05 | 45.14 | ||
| 125 | 23.61 ± 2.78 | 36.75 | ||
| 62.5 | 26.37 ± 3.31 | 29.36 | ||
| 31.25 | 26.91 ± 2.28 | 27.91 | ||
| 15.625 | 29.05 ± 4.01 | 22.18 | ||
| M.Al/L | 1000 | 10.84 ± 0.63 | 70.96 | 198.93 |
| 500 | 13.01 ± 1.83 | 65.15 | ||
| 250 | 17.21 ± 1.50 | 53.90 | ||
| 125 | 20.06 ± 0.27 | 46.26 | ||
| 62.5 | 27.67 ± 0.75 | 25.88 | ||
| 31.25 | 28.32 ± 1.05 | 24.14 | ||
| 15.625 | 30.05 ± 1.61 | 19.50 | ||
| M.Al/B | 1000 | 21.11 ± 2.10 | 43.45 | 3283.28 |
| 500 | 23.65 ± 2.91 | 36.65 | ||
| 250 | 25.98 ± 1.54 | 30.40 | ||
| 125 | 27.76 ± 2.27 | 25.64 | ||
| 62.5 | 29.65 ± 1.79 | 20.57 | ||
| 31.25 | 31.23 ± 2.26 | 16.34 | ||
| 15.625 | 32.01 ± 1.92 | 14.25 | ||
| P.Ha/L | 1000 | 13.98 ± 0.98 | 62.55 | 422.269 |
| 500 | 17.79 ± 1.57 | 52.34 | ||
| 250 | 19.96 ± 1.43 | 46.53 | ||
| 125 | 26.21 ± 1.49 | 29.79 | ||
| 62.5 | 29.14 ± 2.97 | 21.94 | ||
| 31.25 | 30.05 ± 1.77 | 19.50 | ||
| 15.625 | 31.85 ± 1.03 | 14.68 | ||
| P.Ha/S | 1000 | 1.87 ± 0.57 | 94.99 | 52.278 |
| 500 | 4.72 ± 0.86 | 87.36 | ||
| 250 | 10.04 ± 0.45 | 73.10 | ||
| 125 | 13.45 ± 1.60 | 63.97 | ||
| 62.5 | 15.3 ± 1.22 | 59.01 | ||
| 31.25 | 21.93 ± 1.81 | 41.25 | ||
| 15.625 | 27.04 ± 0.30 | 27.56 | ||
| P.Ha/B | 1000 | 24.29 ± 1.25 | 34.93 | 4617.86 |
| 500 | 25.23 ± 2.06 | 32.41 | ||
| 250 | 26.72 ± 1.73 | 28.42 | ||
| 125 | 30.06 ± 3.06 | 19.47 | ||
| 62.5 | 32.62 ± 4.29 | 12.62 | ||
| 31.25 | 34.31 ± 4.20 | 8.09 | ||
| 15.625 | 36.52 ± 2.84 | 2.17 | ||
| P.Ha/R | 1000 | 8.13 ± 0.58 | 78.22 | 150.196 |
| 500 | 13.44 ± 1.59 | 64.00 | ||
| 250 | 15.39 ± 0.14 | 58.77 | ||
| 125 | 17.66 ± 1.39 | 52.69 | ||
| 62.5 | 25.83 ± 0.32 | 30.81 | ||
| 31.25 | 26.97 ± 1.16 | 27.75 | ||
| 15.625 | 30.03 ± 0.90 | 19.56 |
(*mean of twelve replicates).
Where M.Az/F = Melia azedarach (fruit); M.Az/L = Melia azedarach (Leaves); M.Al/L = Morus alba (Leaves); M.Al/B = Morus alba (Branches); P.Ha/L = Peganum harmala (Leaves); P.Ha/S = Peganum harmala (Seeds); P.Ha/B = Peganum harmala (Branches); P.Ha/R = Peganum harmala (Roots).