Figure 3.
Reduced muscle performance was observed in dystrophin-deficient myotubes at day 16 in the short-term training model using three donor-derived iPSCs
(A–C) Functional analyses and Ca2+ peak assay by the SI8000 system of differentiated and mature myotubes from three donor-derived iPSCs on day 16. (A) 409B2, (B) DMDΔ44, and (C) DMDΔ46-47 are shown.
(D–F) qRT-PCR analyses of inflammatory response-related genes on day 16. (D) IL-1β, (E) TNF-α, and (F) IL-6 are shown.
(G) ELISA of IL-6 protein levels in conditioned media harvested on day 16.
(H) An assay of active caspases 8 and 9 on day 16.
(I) cAMP level assay on day 16.
Data represent the mean ± SD and were analyzed by an unpaired t test from five biological replicates (A–C) and three biological replicates (D–I). ∗p < 0.05. N.D., non-detectable.