Figure 4.

A gradual decline in DMD contractile performance was observed in dystrophin-deficient myotubes in the long-term training model using three donor-derived iPSCs

(A) A schematic diagram of the EFS-based long-term training model.

(B) Quantitative time-course analyses of the contraction velocity using the SI8000 motion imaging system.

(C–E) qRT-PCR and ELISA analyses of inflammatory response-related genes on day 28. Both cells and conditioned medium were harvested at day 28. (C) 409B2, (D) DMDΔ44, and (E) DMDΔ46–47 are shown.

(F) Ca²⁺ mobilization assay at day 28.

(G) The cAMP level assay at day 28.

Data represent the mean ± SD and were analyzed using an unpaired t test from at least three biological replicates. ∗p < 0.05.