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. 2021 Jun 4;2(6):100298. doi: 10.1016/j.xcrm.2021.100298
REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies

MHC antibody (MF20) R&D MAB4470; RRID: AB_1293549
FASR/MYH1&2 antibody (MY-32) Sigma M4276; RRID: AB_477190
MYH2 antibody Millipore MABT840
MYH3 antibody Atlas antibodies A75757
MYH7 antibody (A4.840) Santa Cruz sc-53089; RRID: AB_2147281
MYH8 antibody Novus Biologicals NBP2-41309
Sarcomeric α-actinin Abcam ab90776; RRID: AB_10670308
Dystrophin antibody (rod-domain) Leica NCL-DYS1; RRID: AB_442080
RYR1 antibody (D4E1) Cell Signaling Technology 8153; RRID: AB_2797637
Myogenin antibody (F5D) Santa Cruz sc-12732; RRID: AB_627980
TBP antibody (D5C9H) Cell Signaling Technology 44059; RRID: AB_2799258

Chemicals, peptides, and recombinant proteins

StemFit AK02N Reprocell ECAK02N
Primate ES Cell Medium Reprocell RCHEMD001A
Knockout serum replacement Thermo Fisher 10828028
αMEM Nacalai 21444-05
Easy iMatrix-511 Nacalai 892011
Matrigel growth factor reduced BD 356230
Collagen solution Nippi 891500
Blasticidin Wako 026-18711
Puromycin Sigma P8833
Y-27632 Wako 034-24024
Cal-520 AAT Bioquest 21130
Insulin Wako 093-06351
Retinoic acid Sigma R2625
Thyroid hormones Sigma T6397
SB431542 Wako 198-16543
Accutase Nacalai AT104
Doxycycline Nacalai 19088-71
Horse serum Sigma H1138
2-mercaptoethanol Sigma 21985023
Glucose Invitrogen A24940-10
Blocking one Nacalai 03953-95
RIPA buffer Pierce 89900
A complete mini protease inhibitor cocktail Roche 1183617001
FluoroBrite DMEM Invitrogen A18967-01

Critical commercial assays

RNeasy mini kit QIAGEN 74104
PrimeScript RT reagent kit Takara RR037
BAC protein assay kit Pierce 23228
IL-6 Quantikine ELISA kit R&D D6050
Caspase-Glo 8 Promega G8200
Caspase-Glo 9 Promega G8210
LANCE Ultra cAMP Detection kit PerkinElmer TRF0262

Experimental models: Cell lines

DMDΔ44 hiPSC line Shoji et al.21 Shoji et al.21
DMDΔ46-47 hiPSC line Shoji et al.21 Shoji et al.21
409B2 hiPSC line Okita et al.71 Okita et al.

Software and algorithms

GraphPad Prism 7.0e GraphPad N/A
Image Lab Bio-Rad N/A
Microsoft Excel Microsoft N/A
Microsoft Word Microsoft N/A
Adobe Photoshop Adobe N/A
Adobe Illustrator Adobe N/A