Antibodies |
|
MHC antibody (MF20) |
R&D |
MAB4470; RRID: AB_1293549
|
FASR/MYH1&2 antibody (MY-32) |
Sigma |
M4276; RRID: AB_477190
|
MYH2 antibody |
Millipore |
MABT840 |
MYH3 antibody |
Atlas antibodies |
A75757 |
MYH7 antibody (A4.840) |
Santa Cruz |
sc-53089; RRID: AB_2147281
|
MYH8 antibody |
Novus Biologicals |
NBP2-41309 |
Sarcomeric α-actinin |
Abcam |
ab90776; RRID: AB_10670308
|
Dystrophin antibody (rod-domain) |
Leica |
NCL-DYS1; RRID: AB_442080
|
RYR1 antibody (D4E1) |
Cell Signaling Technology |
8153; RRID: AB_2797637
|
Myogenin antibody (F5D) |
Santa Cruz |
sc-12732; RRID: AB_627980
|
TBP antibody (D5C9H) |
Cell Signaling Technology |
44059; RRID: AB_2799258
|
|
Chemicals, peptides, and recombinant proteins |
|
StemFit AK02N |
Reprocell |
ECAK02N |
Primate ES Cell Medium |
Reprocell |
RCHEMD001A |
Knockout serum replacement |
Thermo Fisher |
10828028 |
αMEM |
Nacalai |
21444-05 |
Easy iMatrix-511 |
Nacalai |
892011 |
Matrigel growth factor reduced |
BD |
356230 |
Collagen solution |
Nippi |
891500 |
Blasticidin |
Wako |
026-18711 |
Puromycin |
Sigma |
P8833 |
Y-27632 |
Wako |
034-24024 |
Cal-520 |
AAT Bioquest |
21130 |
Insulin |
Wako |
093-06351 |
Retinoic acid |
Sigma |
R2625 |
Thyroid hormones |
Sigma |
T6397 |
SB431542 |
Wako |
198-16543 |
Accutase |
Nacalai |
AT104 |
Doxycycline |
Nacalai |
19088-71 |
Horse serum |
Sigma |
H1138 |
2-mercaptoethanol |
Sigma |
21985023 |
Glucose |
Invitrogen |
A24940-10 |
Blocking one |
Nacalai |
03953-95 |
RIPA buffer |
Pierce |
89900 |
A complete mini protease inhibitor cocktail |
Roche |
1183617001 |
FluoroBrite DMEM |
Invitrogen |
A18967-01 |
|
Critical commercial assays |
|
RNeasy mini kit |
QIAGEN |
74104 |
PrimeScript RT reagent kit |
Takara |
RR037 |
BAC protein assay kit |
Pierce |
23228 |
IL-6 Quantikine ELISA kit |
R&D |
D6050 |
Caspase-Glo 8 |
Promega |
G8200 |
Caspase-Glo 9 |
Promega |
G8210 |
LANCE Ultra cAMP Detection kit |
PerkinElmer |
TRF0262 |
|
Experimental models: Cell lines |
|
DMDΔ44 hiPSC line |
Shoji et al.21
|
Shoji et al.21
|
DMDΔ46-47 hiPSC line |
Shoji et al.21
|
Shoji et al.21
|
409B2 hiPSC line |
Okita et al.71
|
Okita et al. |
|
Software and algorithms |
|
GraphPad Prism 7.0e |
GraphPad |
N/A |
Image Lab |
Bio-Rad |
N/A |
Microsoft Excel |
Microsoft |
N/A |
Microsoft Word |
Microsoft |
N/A |
Adobe Photoshop |
Adobe |
N/A |
Adobe Illustrator |
Adobe |
N/A |