Figure 3.

Phylogenetic relationships among Blastocystis barcoding region sequences generated in the present study (novel subtypes are represented with a black filled circle and other subtypes with an unfilled circle) and representative reference sequences of all accepted subtypes. Proteromonas lacertae was used as outgroup taxon to root the tree. Analysis was conducted by a neighbor-joining method. Genetic distances were calculated using the Kimura two-parameter model. This analysis involved 70 nucleotide sequences, and there were a total of 590 positions in the final dataset. Bootstrap values lower than 50% are not displayed.