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. 2021 Jun 21;9(6):1343. doi: 10.3390/microorganisms9061343

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Phylogenetic relationships among Blastocystis Santin region sequences generated in the present study (novel subtypes represented are with a black filled circle and other subtypes with an unfilled circle) and representative reference sequences of all accepted subtypes. Proteromonas lacertae was used as outgroup taxon to root the tree. Analysis was conducted using a neighbor-joining method. Genetic distances were calculated using the Kimura two-parameter model. This analysis involved 70 nucleotide sequences, and there were a total of 571 positions in the final dataset. Bootstrap values lower than 50% are not displayed.