Table 1.
Antigenic tests to estimate the toxicity of B. longum/S-eCD.
| (a) ASA Reaction | ||||||||
| Group | Sensitized Antigen | Cause Antigen | No.of Animals | Antigen Challenge Outcome | ||||
| (-) | (+/-) | (+) | (++) | (+++) | ||||
| A | B.longum/S-eCD | B.longum/S-eCD | 5 | 4 | 1 | 0 | 0 | 0 |
| B | B.longum/S-eCD + FCA | B.longum/S-eCD | 5 | 5 | 0 | 0 | 0 | 0 |
| C | OVA + FCA | OVA | 5 | 0 | 0 | 5 | 5 | 4 |
| D | Saline + FCA | B.longum/S-eCD | 5 | 5 | 0 | 0 | 0 | 0 |
| (b) PCA Reaction | ||||||||
| Group | Sensitized Antigen | Cause Antigen | No.of Animals | PCA Titer | ||||
| (×1) | (×4) | (×16) | (×64) | (×256) | ||||
| A | B.longum/S-eCD | B.longum/S-eCD | 5 | 0 | 0 | 0 | 0 | 0 |
| B | B.longum/S-eCD + FCA | B.longum/S-eCD | 5 | 3 | 2 | 2 | 2 | 2 |
| C | OVA + FCA | OVA | 5 | 5 | 5 | 5 | 5 | 4 |
| D | Saline + FCA | B.longum/S-eCD | 5 | 0 | 0 | 0 | 0 | 0 |
Note: (a)Actively immunized guinea pigs were injected intravenously with B. longum/S-eCD or OVA 14 days after the final sensitization. Anaphylaxis symptoms were quantified by the following criteria: –, no symptoms; ±, scrub of face or ear and/or scratch of nose; +, coughing or locomotion ataxia; ++, convulsion or roll, but no death observed within 1 h; and +++, death observed within 1 h. (b) In the PCA reaction, immunized guinea pigs were killed and blood samples were collected 14days after final sensitization to obtain each antiserum. Normal guinea pigs were shaved, and 0.05 mL of each serum dilution was injected intradermally into the dorsal skin. After 4 h, the animals were injected i.v. with 1 mL of antigen (B. longum/S-eCD or OVA) and 0.5 mL of 1% Evans blue solution. After 30 min, the animals were killed, the dorsal skin was peeled off, and blue spots within the intradermal sites were measured. A PCA reaction was judged to be positive when the blue spot measured more than 5 mm2 in dimension. This table was adopted from a previous study [36].