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Alignment of amino acid sequences of PD-L1 from humans with other species. A stalk region of 10–12 amino acids (in bold blue) is located between the IgC-like domain and the transmembrane domain. This region would correspond to the site of cleavage of mPD-L1 by proteases, MMPs and/or ADAMs, to release sPD-L1 [83]. Amino acid sequences were derived from [86,87].
