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. 2021 Jun 21;12(6):948. doi: 10.3390/genes12060948

Figure 1.

Figure 1

Expression of α-synuclein in dopaminergic neurons through Cre-DIO system. AAV containing the double floxed inverted open reading frame (DIO) of the α-synuclein (αSyn) construct, tagged with a V5 epitope at the N-terminal, was administered to the nigra of DAT-Cre rats, which constitutively express Cre recombinases driven by the promoter of dopamine transport (DAT) in dopaminergic neurons. DAT-specific Cre recombinase reverses the gene orientation of V5-αSyn in dopaminergic neurons via action on the lox2272 and loxP. Selective expression of V5-tagged αSyn can, thus, be established in nigral dopaminergic neurons of DAT-Cre transgenic rats. ITR, inverted terminal repeats; hGH poly A, human growth hormone polyadenylation signal; WPRE, woodchuck hepatitis virus post-transcriptional regulatory element.