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αSyn is expressed in nigral TH neurons of DAT-Cre rats receiving AAV-DIO-αSyn. DAT-Cre rats were stereotaxically injected with AAV-DIO-αSyn to SNcd. Brain tissues were collected for TH and V5-αSyn immunostaining. (A) At lower magnification, TH and V5-αSyn were present in the nigra region. (B) At higher magnification, almost all V5-αSyn (+) cells co-expressed TH immunoreactivity (arrows) in the SNcd.
