Figure 6.

The EPAC2-mediated NF-κB activation by poly I:C or TNF-α. (A,B) 293 cells in triplicate were transiently transfected with a luciferase reporter plasmid containing the NF-κB promoter (NF-κB-luc) in the presence of the indicated drugs. At 15 h post-transfection, cells were transfected with 1 μg/mL poly I:C (A) or treated with 10 ng/mL of TNF-α (B) for an additional 6 h. Cells were then lysed to measure luciferase activities. Cells without poly I:C or TNF-α treatment were used as a negative control. Data are representative of three independent experiments. * p < 0.05 and ** p < 0.01, relative to the poly I:C-transfected and DMSO-treated group or the TNF-α- and DMSO-treated group, respectively.