Skip to main content
. 2021 Jun 16;13(6):892. doi: 10.3390/pharmaceutics13060892

Figure 5.

Figure 5

BBB phenotype comparison between the original 12 TW model and the miniaturized 96 TW system. (A) Endothelial permeability coefficient (Pe in cm/min) measuring the transport of sodium fluorescein (NaF) through the BLECs in the original 12 TW model and the miniaturized 96 TW system over the different cell seeding series. The results are presented in a scatter plot for the original 12 TW models: n = 57, N = 19 and 96 TW systems: n = 194, N = 22 (unpaired t-test; ns = no significant differences). (B) Immunocytochemical CD34 +-ECs BBB phenotype validated by the expression of the proteins involved in the junctions (in green): VE cadherin, PECAM (CD31+), Zonula occludens-1 and Claudin-5. Nuclei are in blue. Original 12 TW images were taken from the luminal BLEC face, while images in the 96 TW system were taken from the abluminal BLEC face, using the ImageXpress Micro Confocal High-Content Imaging System, objective 20× (scale bar = 25 µm). (C) Assessment of the efflux pump functionality. Percentage of the R123 intracellular accumulation in the BLECs in the presence or absence of the inhibitor Elacridar (GF) in the original model and in the miniaturized model. Samples: n = 9, N = 3 (multiple t-test; * p-value < 0.05).