Table 2.
Descriptive and comparative summaries of the human BBB in vitro model development and processing: original 12-well TW model and the miniaturized and automated 96 TW system replicates.
| BBB In Vitro System | Original 12 TW Model | 96 TW Systems | |
|---|---|---|---|
| Brand | Corning | Falcon | Corning |
| Cell filter growth area | 1.13 cm2 | 0.0804 cm2 | 0.143 cm2 |
| Manual cell seeding ratio number (PCs well//ECs filter) | ECs 80,000//PCs 50,000 | ECs 18,000//PCs 15,000 | ECs > 15,000//PCs 15,000 |
| Automated cell seeding ratio (PCs well//ECs filter) | - | ECs 18,000//PCs 15,000 | ECs 22,000//PCs 15,000 |
| Filters adaptation for cell visualization by Confocal Microscopy | Cut and placed in cover slip | 96 TW ready for automation | 96 TW ready for automation |
| Bottom plate adapted for cell visualization | Yes | No | Yes |
| Permeability/staining assays | Manually | Automated | Automated |