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. 2021 Jun 22;15(3):158–166. doi: 10.22074/IJFS.2021.134602

Table 1.

Gene expression analysis in sperm, testis tissue and semen of fertile and infertile men


Gene name Function Sample type AssociationP value Ref.

DNMT1, DNMT3A, and DNMT3B Methylation of DNA Semen No (7)
RXFP3 Peptide receptor Spermatozoa No (8)
PLCζ Phospholipase (tes‌tis-specific) Semen Yes (9)
PLCζ Phospholipase (tes‌tis-specific) Sperm YesP≤0.05 (10)
PLCζ and CAPZA3 Phospholipase (tes‌tis-specific)/F-actin capping protein Semen Yes (11)
PLCζ and PAWP Phospholipase (tes‌tis-specific)/Meiotic resumption Semen Yes (12)
PLCζ, PAWP and TR-KIT Phospholipase (tes‌tis-specific)/Meiotic resumption/KIT proto-oncogene receptor tyrosine kinase Semen Yes (13)
PAWP Meiotic resumption Semen YesP<0.05 (14)
TR-KIT KIT proto-oncogene receptor tyrosine kinase Semen YesP<0.01 (15)
JMJD1A Demethylase Tes‌tis tissue Yes (16)
PRM1, PRM2, YBX2 and JHDM2A Compact sperm DNA (tes‌tis-specific)/DNA- RNA-binding protein (tes‌tis-specific)/Demethylase Tes‌tis tissue Yes/No for JHDM2A (17)
YBX2 and JHDM2A DNA- RNA-binding protein (tes‌tis-specific)/Demethylase Tes‌tis tissue Yes/No for JHDM2A (18)
YBX2 DNA-RNA-binding protein (tes‌tis-specific) Tes‌tis tissue Yes P<0.0001 (19)
PRM1, PRM2 and TNP2 Compact DNA sperm (tes‌tis-specific )/Replacement of his‌tones to protamine (tes‌tis-specific) Semen PRM1, PRM2decrease/TNP2 increase (20)
PRM1 and PRM2 Compact DNA sperm (tes‌tis-specific) Tes‌tis tissue Yes, for PRM1 P<0.001 (21)
PRM2 Compact sperm DNA (tes‌tis-specific) Semen No (22)
Casp 9 and PRM2 Apoptosis/Compact sperm DNA (tes‌tis-specific) Semen Yes, for PRM2 P<0.05 (23)
KDM3A and PRM1 Demethylase/Compact sperm DNA (tes‌tis-specific) Tes‌tis tissue Decrease in NOA (24)
DAZ, AKAP4, PRM1 and PRM2 RNA-binding protein/Regulatory subunit of protein kinase A/Compact DNA sperm (tes‌tis-specific) Semen Yes, for DAZ and PRM2 (5)
PRM1 PRM2 and HILS1 Compact DNA sperm (tes‌tis-specific)/Linker his‌tone Sperm Yes, for PRM1 and PRM2 P<0.001 (25)
ZMYND15, TNP1, PRM1 and SPEM1 Transcriptional repressor/Replacement of his‌tones to protamine (tes‌tis-specific)/Compact sperm DNA (tes‌tis-specific)/Spermatid maturation (tes‌tis-specific) Tes‌tis tissue Yes (26)
PRM2, HSP90 and WNT5A Compact sperm DNA (tes‌tis-specific)/Chaperone/Signaling proteins Sperm Yes P≤0.05 (27)
TNP1 Replacement of his‌tones to protamine (tes‌tis-specific Semen Yes --- (28)
HSPA2 Folding and transport Semen No (29)
TGIFLX/Y Transcription factor (tes‌tis-specific) Tes‌tis tissue Yes (30)
SYCP3 Recombination Tes‌tis tissue Yes (31)
Septin14 GTP-binding cytoskeletal proteins Tes‌tis tissue Yes (32)
DAZ RNA-binding protein Tes‌tis tissue Yes (33)
TSGA10 Sperm tail fibrous sheath Tes‌tis tissue Yes (34)
Clus‌terin Chaperone Tes‌tis tissue Yes (35)
hTSH2B His‌tone Tes‌tis tissue Yes (36)
BAX and BCL-2 Apoptotic regulators Semen No/Yes (37)
ERα Es‌trogen receptor Sperm Yes P≤0.05 (38)
Semen Yes (39)
SREs Sperm RNA elements Sperm P≤0.05 (40)