Figure 1.

Response of Arabidopsis srfr1 mutants to the necrotrophic fungal pathogen, Fusarium oxysporum. (a) Infection with conidia and mycelial plugs on Arabidopsis RLD, srfr1-1, and srfr1-2 mutants. Detached leaves from 4-week-old plants were inoculated with 4 mm-diameter plugs of F. oxysproum. Photographs were taken at 14 DPI; (b) box plots of lesion size at 14 DPI in Col-0, RLD, srfr1-1, and srfr1-2. The y-axis displays the measured diameter of disease lesion (mm, n = 12) in each plant. The box ranges were determined from the twenty-fifth to the seventy-fifth percentiles. A statistically significant difference was determined by the Student’s t-test (* p < 0.05); (c) Leaves were collected 14 DPI with F. oxysporum and stained with lacto-phenol trypan blue to visualize the extent of hyphal development. The upper panel represents destained leaves with 99% ethanol, and the lower panel indicates developed fungal hyphae in each plant. Scale bar: 200 μm. This experiment was repeated once with similar results.