Figure 2.

Response of Arabidopsis srfr1 mutants to the necrotrophic fungal pathogen, Botrytis cinerea. (a) Fungal spores of B. cinerea grown on PDA were harvested and inoculated onto detached leaves of Arabidopsis RLD, srfr1-1, and srfr1-2 mutants at a concentration of 1 × 10⁸ spores/mL. Photographs were taken at 5 DPI; (b) box plots of lesion size at 5 DPI in RLD, srfr1-1, and srfr1-2. The y-axis displays the measured diameter of disease lesions (mm, n = 12) in each plant. The box ranges were determined from the twenty-fifth to the seventy-fifth percentiles. A statistically significant difference was determined by the Student’s t-test (* p < 0.01); (c) leaves were collected 3 DPI with B. cinerea and stained with lacto-phenol trypan blue to visualize the extent of hyphal development. Scale bar: 200 μm.