Figure 5.

ICE analysis with amplified PCR fragments from slsrfr1 G1 plants, Sanger sequencing chromatograms in target sequence, and predicted amino acid sequences in two homozygous G1-slsrfr1 plants. (a) Eleven nucleotide deletions in slsrfr1-1 produces a premature stop codon, colored with blue; (b) 1 or 4 nucleotide deletions in slsrfr1-2 remove a start codon (M), colored with red. Nucleotide deletions are indicated by dashes (-). The PAM sites are boxed, and the target sequences of guide RNAs are overlined.