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. 2021 Jun 15;22(12):6427. doi: 10.3390/ijms22126427

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Altered morphology and expression of defense marker genes in CRISPR/Cas9-edited slsrfr1 plants. (a) Growth phenotype of 6-week-old M82 and slsrfr1 lines grown in 16 h light/8 h dark long-day photoperiod; (b) relative mRNA expression of defense-related genes. The genes used for gene expression analysis refer to SlPR1 (Solyc09g007010.1), SlPR2 (Solyc01g008620.2), SlPR5(Solyc08g080640), and TomloxD (Solyc03g122340.2). Gene expression levels of each gene were normalized with SlACT (Solyc04g011500.3.1) as an internal control. Error bars represent standard deviation. A statistically significant difference was determined by the Student’s t-test (* p < 0.01).