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. 2021 Jun 16;22(12):6431. doi: 10.3390/ijms22126431

Figure 1.

Figure 1

SOD3 and SOD3-MSCs inhibit the MAPK activation and inflammatory responses in response to TNF-α treatment. SOD3 (300 unit/mL) pretreatment or co-culture with SOD3-MSCs or MSCs were conducted to determine inflammatory activation of Caco-2 cells treated with TNF-α (100 ng/mL). (A) Schematic diagram of in vitro experiments. After harvesting Caco-2 cells, RNA or protein was extracted for further analysis. (B) The expression of p-JNK, JNK, p-ERK1/2, and ERK1/2 was determined by western blotting. (C) The expression of pro- (IL-6, IL-8, IL-1β, IL-1 and IL-17C) or anti-inflammatory (IL-10) cytokines in mRNA level was determined by quantitative PCR. CTL: untreated control; P.C: TNF-α-treated positive control, ## p < 0.01, ### p < 0.001 (CTL vs. P.C), ** p < 0.01, *** p < 0.001 (P.C vs. SOD3, SOD3-MSC and MSCs), $ p < 0.05, $$ p < 0.01, $$$ p < 0.001 (SOD3, SOD3-MSCs vs. MSCs). Results are shown as mean ± SD. Results show one representative experiment of at least three independent experiments.