Figure 6.

The protective action of SOD3 protein on IOs against pro-inflammatory and oxidative stress. (A,B) The representative bright-field images and live/dead assessment of IFN-γ (A) or TNF-α (B) treated IOs on culture day 5. Cytokines were added on culture day 3. (C) To induce the generation of reactive oxygen species, tBHP was added to IO culture then their mitochondrial activity was determined by TMRE staining. Mean fluorescence intensity (MFI) of CTL was standardized as 1. At least three individual experiments were performed. * p < 0.05, ** p < 0.01, *** p < 0.001 (CTL vs. IFN-γ and TNF-α in A,B; CTL vs. others in C), # p > 0.05 (TNF-α vs. SOD3 protein). Results are shown as mean ± SD. Results show one representative experiment of at least three independent experiments.