Table 1.
Median methylation values of cases and controls by pyrosequencing.
| Median | Man-Whitney (Wilcoxon Rank Sum) | ROC Curve | 95% CI Interval | Univariate Regression High HPV Methylation vs. Low/Medium Methylation | ||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Region Name | Genome Position | Control (n = 39) |
Case (n = 50) |
Difference | p Value | AUC | OR | 95% CI | p Value | |
| E7 | c_754 c_776 c_779 c_787 |
1.40 13.20 1.20 5.90 |
1.80 13.30 1.20 6.40 |
0.40 0.10 0.00 0.50 |
0.010910 0.542481 0.771323 0.141489 |
0.6583 0.5379 0.5181 0.5916 |
(0.54, 0.78) (0.42, 0.66) (0.39, 0.65) (0.47, 0.71) |
4.64 2.36 0.50 1.99 |
(1.88, 11.47) (0.95, 5.89) (0.18, 1.39) (0.82, 4.81) |
0.000886 0.064880 0.182842 0.125934 |
| E1 | c_1122 c_1141 |
1.85 6.20 |
1.55 7.55 |
−0.30 1.35 |
0.319005 0.057360 |
0.4405 0.6187 |
(0.32, 0.56) (0.50, 0.74) |
0.61 1.85 |
(0.23, 1.59) (0.76, 4.46) |
0.313096 0.173033 |
| L2 | c_4274 c_4283 c_4292 c_4919 c_4926 c_4931 c_4949 |
8.00 18.50 10.90 2.40 4.30 4.40 32.60 |
13.50 17.20 19.90 3.00 5.25 4.70 39.50 |
5.50 −1.30 9.00 0.60 0.95 0.30 6.90 |
0.001171 0.003156 0.001699 0.085078 0.251942 0.295395 0.371808 |
0.7013 0.6831 0.6946 0.6062 0.5710 0.5649 0.5554 |
(0.59, 0.82) (0.57, 0.80) (0.58, 0.81) (0.49, 0.72) (0.45, 0.69) (0.45, 0.68) (0.43, 0.68) |
2.44 2.25 2.44 2.25 1.50 1.56 1.38 |
(1.01, 5.86) (0.94, 5.41) (1.01, 5.86) (0.94, 5.41) (0.62, 3.63) (0.63, 3.84) (0.57, 3.35) |
0.046613 0.070045 0.046613 0.070045 0.368990 0.333509 0.478098 |
| L1 | c_5606 c_5609 c_5615 c_5621 c_5648 c_5655 c_7098 c_7111 c_7119 |
18.00 15.00 30.20 5.00 26.80 35.90 3.10 5.90 3.40 |
33.20 25.90 48.10 8.05 32.15 51.20 3.90 10.30 5.70 |
15.20 10.90 17.90 3.05 5.35 15.30 0.80 4.40 2.30 |
0.001393 0.007199 0.000257 0.001651 0.034620 0.001799 0.024831 0.016845 0.032968 |
0.6982 0.6667 0.7267 0.6951 0.631 0.6936 0.6397 0.6489 0.6327 |
(0.58, 0.81) (0.55, 0.79) (0.62, 0.83) (0.58, 0.81) (0.51, 0.75) (0.58, 0.80) (0.52, 0.76) (0.53, 0.77) (0.51, 0.76) |
3.38 2.64 4.78 2.86 2.08 3.67 1.99 2.76 3.55 |
(1.39, 8.17) (1.10, 6.36) (1.94, 11.80) (1.19, 6.90) (0.86, 5.00) (1.51, 8.92) (0.82, 4.81) (1.14, 6.68) (1.46, 8.65) |
0.007037 0.030232 0.000684 0.019101 0.102620 0.004099 0.125934 0.024124 0.005258 |
| URR | c_7557 c_7563 |
2.50 2.80 |
2.50 3.05 |
0.00 0.25 |
0.885024 0.423051 |
0.5096 0.553 |
(0.37, 0.64) (0.42, 0.68) |
0.81 1.71 |
(0.30, 2.19) (0.68, 2.01) |
0.680344 0.579475 |
Note: Pyrosequencing was conducted for cases (n = 50) and controls (n = 39) across 24 CpG loci, as described in the Materials and Methods section. Methylated loci are grouped to their location within the HPV52 genome.