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. 2021 Jun 21;13(12):3084. doi: 10.3390/cancers13123084

Figure 6.

Figure 6

Crosslinked selicrelumab can sensitize CLL cells for cell death induced by anti-CD20 mAb GA101, as well as for the Fc mutated version GA101-P329GLALA. A,B,C CLL cells were cultured and CD40 stimulated as previously described for 48 h. (a) After stimulation, cells were incubated with GA10 for 24 h, viability was measured as previously described and specific apoptosis was calculated (n = 22). Results revealed no differences between IGHV mutated and unmutated patients (non-significant for 3T40L, aCD40 and aCD40XL stimulation). (b) Stimulated CLL cells were incubated in the presence/absence of bafilomycin for 1 h and treated with GA101 for 24 h (n = 7 ≤ 9). (c) Stimulated CLL cells were incubated with GA101 and GA101-P329GLALA in the presence of specific crosslinker reagent TN86 for 24 h (n = 5 ≤ 8). Bars represent the mean ± SEM: * p <0.05, ** p <0.01, **** p <0.0001 (unpaired t-test).