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. 2021 Jun 19;22(12):6580. doi: 10.3390/ijms22126580

Figure 3.

Figure 3

Focal adhesions maintain response to mechanical stimulus in lamin A/C depleted MSCs: (a) Representative western blots for pFAK (Tyr 397), TFAK, and lamin A/C in siCntl and siLmna treated cells groups treated with 2 bouts of LIV (20 min, 90 Hz, 0.7 g) separated by 2 hour rest period. LIV treated samples had a 2-fold increase of pFAK compared to non-LIV. (b) Analysis of western image of pFAK, TFAK, and lamin A/C during LIV (n = 4/grp). The non-LIV siLlmna group had a 92% (p < 0.05) increased basal pFAK compared to the non-LIV siCntl group. In response to LIV, both siCtnl and siLmna treated MSCs elicited 101% (p < 0.05) and 87% (p < 0.001) increases in pFAK, respectively, compared to non-LIV controls. (c) Representative western blots for pFAK (Tyr 397), TFAK, and Lamin A/C of the siCntl and siLmna groups treated with a single bout strain (20 min, 0.1 Hz, 2% strain). (d) Analysis of pFAK, TFAK, and lamin A/C immediately after strain application (n = 4/grp). The non-strain siLmna group had a 79% (p < 0.05) increased basal pFAK compared to the non-strain siCntl group. In response to strain, pFAK levels were elevated by 331% (p < 0.001) and 83% (p < 0.001) in siCtnl and siLmna treated MSCs respectively. Results are presented as mean ± STE. Group comparisons were made via one-way ANOVA followed by a Newman-Keuls post-hoc test. * p < 0.05, *** p < 0.001, against control or against each other.