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. 2021 Jun 21;10(12):2735. doi: 10.3390/jcm10122735

Figure 1.

Figure 1

Real-time polymerase chain reaction results of the patient with positive SARS-CoV-2 in the vaginal secretions. (A) Results from nasopharyngeal swab utilizing the DiaSorin Molecular Simplexa™ COVID-19 Direct real-time RT-PCR assay, testing for genes S and Orf1b. (B) Results from the first positive vaginal swab utilizing the DiaSorin Molecular Simplexa™ COVID-19 Direct real-time RT-PCR assay, testing for genes S and Orf1b. (C) Results from the repeat vaginal swab utilizing GeneXpert® Xpress SARS-CoV-2, testing for genes E and N2. Cycle threshold (Ct) levels are inversely proportional to the amount of mRNA of the targeted antigen. The Ct of the S gene, ORF1, and N2 in the vaginal samples was 34.6, 36.1, and 41.7, respectively (B,C). The X and Y axes represent the amount of amplified RNA and the number of cycles, respectively; the earlier the signal is detected, the lower the number of cycles and the higher the viral load. The orange line (C), sample processing control (SPC), and the purple line (B), RNA internal control (RNA IC), represent the positive controls. A positive result for the genes E and N2 is determined with values of Ct < 45 [22]. Positive results for both methods were determined according to known values [22,23]. Cycle threshold (Ct) levels are inversely proportional to the amount of mRNA of the targeted antigen. Ct of the S gene, ORF1 and N2 in the vaginal samples were 34.6, 36.1 and 41.7, respectively (B,C).