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Co-immunoprecipitation of Sig1R and Nrf2 in 661 W cells. Lysates of 661 W cells were incubated with anti-Sig1R antibody and immune complexes were precipitated by protein A/G beads. The Sig1R complex was subjected to immunoblotting to detect (A) NRF2 and (B) Sig1R in the complex. The whole cell lysate was used for immunoblotting to detect (C) NRF2 and (D) Sig1R. (E) GAPDH served as an internal reference for sample loading.
