Figure 3.

mat K target detection analyzing peanut DNA samples isolated with three different protocols. (A) Calibration curves of real-time PCR assay using mat K specific primers and probe, performed with 10-fold serial diluted peanut DNA isolated by three different isolation protocols (1–3); (B) calibration curves performed with DNA from binary mixtures of peanut in wheat, using DNA obtained with the three different protocols. Efficiency and R² values are included for the two kit-based DNA isolation protocols. Only Ct values obtained after DNA isolation by protocol 3 are shown as grey circles for comparison purposes (samples containing 100, 10, and 1 mg of peanut/kg of mixture). Mean and standard error are represented.