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. 2021 Jun 18;22(12):6542. doi: 10.3390/ijms22126542

Figure 3.

Figure 3

TA and 9-AC prevent current facilitation and inhibit single-channel activity. (A) Whole-cell currents evoked by repeated 2 s applications of 10 μM BzATP in the presence (blue) or absence of 9-AC (grey) in HEK293T cells transiently expressing rP2X7. Currents are normalized to the first application. (B) Summary of currents relative to the first application in the absence (control) or presence of inhibitors (n = 4 cells for TA, 10 for 9-AC, 8 for FFA, and 8 for control). Symbols represent mean ± SEM. (C) Single-channel rP2X7 currents elicited by 10 μM BzATP from an outside-out patch of HEK293T cell (black traces) are inhibited by 1 mM 9-AC when co-applied with 10 μM BzATP (blue traces). Shown sweeps were sequentially recorded from the same patch. Note that inhibition is reversible after washout. Duration of application is indicated by the white bar and grey shading. Data were filtered at a final fc of 100 Hz. (D) Summary of single-channel inhibition (n = 3 patches for TA, 6 for 9-AC, 3 for FFA, and 3 for AZ10606120). Bars represent mean ± SEM. See Materials and Methods for calculation. Inhibitor concentrations were 20 μM TA, 1 mM 9-AC, 100 μM FFA and 1 μM AZ10606120. Single-channel and whole-cell data were recorded at −120 and −60 mV, respectively.