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. 2021 Jun 17;13(6):894. doi: 10.3390/pharmaceutics13060894

Figure 8.

Figure 8

In vitro cytotoxicity of Cu(DDC)2 liposomes on neuroblastoma 2D and 3D cell cultures. (a,b) LS monolayers (2D) and LS monoculture spheroids (3D) were treated 72 h with non-PEGylated (a) and PEGylated (b) Cu(DDC)2 liposomes. 3D spheroids were generated by using agarose-coated wells with subsequent centrifugation. Cell viability curves were obtained using 2D and 3D CellTiter-Glo® assay, respectively after indicated treatment duration. Data are expressed as the mean ± SD (LS, 2D: n = 7; LS, 3D: n = 5). (c,d) EC50 values after 72 h of treatment with non-PEGylated (c) and PEGylated (d) Cu(DDC)2 liposomes. Data are expressed as the mean ± SD (n = 5–7). (e,f) Representative brightfield microscopy images of LS monoculture spheroids. Images were taken after 24, 48, and 72 h of treatment with non-PEGylated (e) and PEGylated (f) Cu(DDC)2 liposomes, respectively. Scale bar = 400 µm.