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In vitro cytotoxicity of Cu(DDC)2 liposomes on 2D hSkMC and 2D hDFa monoculture cell models. (a,b) 2D monolayers of LS, hSkMC, and hDFa cells were incubated with non-PEGylated (a) and PEGylated (b) Cu(DDC)2 liposomes for 24 h. Cell viability was analyzed using the 2D CellTiter-Glo® assay after indicated treatment duration. (c,d) EC50 values after 24 h of treatment with non-PEGylated (c) and PEGylated (d) Cu(DDC)2 liposomes. Data are expressed as the mean ± SD (n = 3).
