Figure 4.

Temperature-inducible EDS1 and PAD4 confer enhanced resistance to bacterial pathogens at elevated temperatures. Growth of bacterial strain Pst DC3000 in leaves of Arabidopsis Col and transgenic lines expressing FLAG-EDS1 and YFP-PAD4 under control of the promoter of HSP70 gene (proHSP70-EP). Plants were grown at 22 °C for 4 weeks, then half of them were shift to 28 °C 24 h before bacterial infection for measuring resistance at an elevated temperature. Plant leaves were hand-infiltrated with bacterial suspensions (OD600 = 0.0002) and bacterial titers were determined at 3 dpi. Error bars represent mean ± SD of six biological replicates. Different letters indicate statistical significance (p < 0.05) determined by one-way ANOVA followed by Tukey’s HSD. The experiment was repeated three times with similar results.