Table 3.
Calcium, phosphorous, and related regulatory hormones and metabolite values measured in blood given as the means and 95% confidence intervals (CI) for the control (n = 8) and test (n = 10) diet dog groups at the baseline and at eight-week intervals. P values represent the comparison between diet groups at the sample collection time point.
| - | Weeks | Control | Test | p | ||
|---|---|---|---|---|---|---|
| Mean | 95% Cl | Mean | 95% Cl | |||
| Plasma calcium (mmol/L) | Baseline | 2.48 | 2.42, 2.54 | 2.40 | 2.35, 2.45 | 0.098 |
| 8 | 2.48 | 2.42, 2.54 | 2.44 | 2.38, 2.49 | 0.757 | |
| 16 | 2.50 | 2.44, 2.56 | 2.44 | 2.39, 2.49 | 0.292 | |
| 24 | 2.53 * | 2.47, 2.59 | 2.46 * | 2.41, 2.52 | 0.245 | |
| 32 | 2.54 * | 2.48, 2.61 | 2.49 * | 2.44, 2.54 | 0.505 | |
| 40 | 2.54 * | 2.48, 2.60 | 2.47 * | 2.42, 2.53 | 0.236 | |
| Plasma phosphorus (mmol/L) | Baseline | 1.24 | 1.11, 1.39 | 1.30 | 1.18, 1.44 | 0.992 |
| 8 | 1.21 | 1.08, 1.36 | 1.20 | 1.08, 1.33 | 1.000 | |
| 16 | 1.29 | 1.15, 1.45 | 1.19 | 1.08, 1.32 | 0.817 | |
| 24 | 1.22 | 1.08, 1.36 | 1.20 | 1.08, 1.33 | 1.000 | |
| 32 | 1.26 | 1.12, 1.41 | 1.14 * | 1.03, 1.26 | 0.558 | |
| 40 | 1.17 | 1.04, 1.31 | 1.15 * | 1.04, 1.27 | 1.000 | |
| Ionized calcium (mmol/L) | Baseline | 1.29 | 1.25, 1.33 | 1.28 | 1.24, 1.32 | 0.999 |
| 8 | 1.18 * | 1.14, 1.22 | 1.29 | 1.26, 1.33 | <0.001 ** | |
| 16 | 1.30 | 1.26, 1.34 | 1.31 | 1.27, 1.34 | 1.000 | |
| 24 | 1.30 | 1.26, 1.34 | 1.32 | 1.28, 1.36 | 0.989 | |
| 32 | 1.31 | 1.26, 1.35 | 1.30 | 1.27, 1.34 | 1.000 | |
| 40 | 1.28 | 1.24, 1.32 | 1.29 | 1.25, 1.32 | 1.000 | |
| Parathyroid hormone (pg/mL) | Baseline | 18.27 | 11.72, 28.47 | 12.18 | 8.19, 18.11 | 0.488 |
| 8 | 12.38 | 7.94, 19.29 | 11.06 | 7.44, 16.45 | 1.000 | |
| 16 | 12.32 | 7.90, 19.19 | 14.13 | 9.50, 21.01 | 1.000 | |
| 24 | 10.33 | 6.63, 16.09 | 9.99 | 6.72, 14.86 | 1.000 | |
| 32 | 10.58 | 6.79, 16.49 | 9.61 | 6.46, 14.29 | 1.000 | |
| 40 | 15.26 | 9.79, 23.78 | 13.72 | 9.23, 20.41 | 1.000 | |
| Fibroblast growth factor 23 (pg/mL) | Baseline | 336 | 253, 447 | 411 | 319, 530 | 0.808 |
| 8 | 351 | 264, 466 | 392 | 304, 505 | 0.996 | |
| 16 | 311 | 234, 414 | 384 | 298, 496 | 0.759 | |
| 24 | 293 | 220, 390 | 348 | 270, 449 | 0.912 | |
| 32 | 323 | 243, 429 | 339 | 263, 438 | 1.000 | |
| 40 | 178 * | 134, 237 | 209 * | 162, 269 | 0.952 | |
| Total calcidiol (25OHD) (nmol/L) | Baseline | 70.30 | 56.97, 86.75 | 79.10 | 65.54, 95.47 | 0.956 |
| 8 | 73.46 | 59.53, 90.65 | 82.98 | 68.76, 100.15 | 0.946 | |
| 16 | 69.37 | 56.21, 85.60 | 79.06 | 65.51, 95.42 | 0.918 | |
| 24 | 75.28 | 61.01, 92.89 | 80.81 | 66.96, 97.53 | 0.999 | |
| 32 | 82.49 * | 66.85, 101.80 | 86.89 | 71.99, 104.87 | 1.000 | |
| 40 | 78.52 | 63.63, 96.89 | 86.44 | 71.63, 104.33 | 0.990 | |
| Calcitriol (1,25(OH)2D) (pmol/L) | Baseline | 25.10 | 18.25, 34.53 | 25.43 | 19.25, 33.60 | 1.000 |
| 8 | 17.49 * | 12.55, 24.38 | 30.40 | 23.01, 40.17 | 0.008 ** | |
| 16 | 16.80 * | 12.05, 23.41 | 27.61 | 20.90, 36.48 | 0.027 ** | |
| 24 | 21.01 | 15.23, 29.00 | 26.72 | 20.22, 35.31 | 0.757 | |
| 32 | 17.96 * | 13.02, 24.79 | 23.38 | 17.61, 31.05 | 0.660 | |
| 40 | 17.80 * | 12.89, 24.56 | 25.17 | 19.05, 33.25 | 0.284 | |
| 24,25-dihydroxycholecalciferol (24,25(OH)2D3) (nmol/L) | Baseline | 31.12 | 23.08, 41.98 | 35.05 | 26.82, 45.80 | 0.997 |
| 8 | 30.84 | 22.87, 41.59 | 33.76 | 25.83, 44.11 | 1.000 | |
| 16 | 25.56 * | 18.95, 34.47 | 31.23 | 23.90, 40.81 | 0.884 | |
| 24 | 23.21 * | 17.21, 31.30 | 26.88 * | 20.57, 35.13 | 0.984 | |
| 32 | 23.48 * | 17.41, 31.66 | 24.22 * | 18.54, 31.65 | 1.000 | |
| 40 | 23.23 * | 17.22, 31.33 | 25.65 * | 19.63, 33.51 | 0.999 | |
| 24,25-dihydroxyergocalciferol (24,25(OH)D2) (nmol/L) | Baseline | 9.19 | 6.33, 13.33 | 7.06 | 5.06, 9.85 | 0.857 |
| 8 | 9.27 | 6.39, 13.45 | 7.89 | 5.66, 11.01 | 0.995 | |
| 16 | 7.57 * | 5.21, 10.98 | 6.74 | 4.83, 9.40 | 1.000 | |
| 24 | 6.77 * | 4.67, 9.83 | 5.97 * | 4.28, 8.32 | 0.999 | |
| 32 | 6.25 * | 4.31, 9.07 | 5.72 * | 4.10, 7.98 | 1.000 | |
| 40 | 6.79 * | 4.68, 9.85 | 5.69 * | 4.08, 7.94 | 0.989 | |
| Total dihydroxycalcidiol (24,25(OH)2D) (nmol/L) | Baseline | 40.83 | 31.33, 53.21 | 42.72 | 33.71, 54.14 | 1.000 |
| 8 | 40.69 | 31.22, 53.03 | 42.53 | 33.56, 53.90 | 1.000 | |
| 16 | 33.58 * | 25.77, 43.77 | 38.57 | 30.44, 48.88 | 0.974 | |
| 24 | 30.51 * | 23.41, 39.77 | 33.45 * | 26.39, 42.39 | 0.999 | |
| 32 | 30.11 * | 23.10, 39.24 | 30.60 * | 24.14, 38.77 | 1.000 | |
| 40 | 30.33 * | 23.27, 39.53 | 31.95 * | 25.21, 40.48 | 1.000 | |
| Calcidiol (25OHD3) (nmol/L) | Baseline | 68.97 | 55.79, 85.26 | 77.59 | 64.19, 93.79 | 0.959 |
| 8 | 72.41 | 58.58, 89.52 | 81.86 | 67.72, 98.95 | 0.947 | |
| 16 | 68.24 | 55.20, 84.36 | 78.10 | 64.60, 94.40 | 0.907 | |
| 24 | 73.12 | 59.15, 90.39 | 79.40 | 65.68, 95.98 | 0.997 | |
| 32 | 78.99 | 63.90, 97.65 | 83.44 | 69.03, 100.87 | 1.000 | |
| 40 | 73.25 | 59.26, 90.56 | 83.73 | 69.27, 101.22 | 0.912 | |
| Ergocalcidiol (25OHD2) (nmol/L) | Baseline | 1.26 | 0.79, 1.99 | 1.47 | 0.97, 2.22 | 0.999 |
| 8 | 1.03 | 0.65, 1.64 | 1.09 | 0.72, 1.64 | 1.000 | |
| 16 | 1.09 | 0.69, 1.72 | 0.92 | 0.61, 1.40 | 0.998 | |
| 24 | 1.85 | 1.17, 2.93 | 1.27 | 0.84, 1.92 | 0.649 | |
| 32 | 2.88 * | 1.81, 4.56 | 2.65 * | 1.75, 4.00 | 1.000 | |
| 40 | 4.34 * | 2.74, 6.89 | 2.51 * | 1.66, 3.80 | 0.165 | |
| Total calcidiol: total dihydroxycalcidiol ratio (25OHD:24,25(OH)2D) | Baseline | 1.72 | 1.49, 1.98 | 1.85 | 1.63, 2.10 | 0.970 |
| 8 | 1.81 | 1.57, 2.08 | 1.95 | 1.72, 2.21 | 0.954 | |
| 16 | 2.07 * | 1.79, 2.38 | 2.05 | 1.81, 2.33 | 1.000 | |
| 24 | 2.47 * | 2.14, 2.84 | 2.42 * | 2.13, 2.74 | 1.000 | |
| 32 | 2.74 * | 2.38, 3.16 | 2.84 * | 2.50, 3.22 | 1.000 | |
| 40 | 2.59 * | 2.25, 2.98 | 2.71 * | 2.38, 3.07 | 0.999 | |
* A significant difference from baseline within diet group (p < 0.05). ** A significant difference from baseline between diet groups (p < 0.05).