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. 2021 Jun 16;11(6):898. doi: 10.3390/biom11060898

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Relative expression of SiTPS when S. indica is grown for 14 days on complete medium (CM) agar plates compared to S. indica colonizing tomato roots (11 days post inoculation, dpi). Relative expression under both conditions was normalized using SiGAPDH (GenBank: FJ810523.1) expression levels and calculated with the 2^−ΔCt method [39,40]. Error bars represent the standard error of the mean (n = 4). The asterisk (*) indicates a significant difference between the two conditions (t-test in R-studio, * p ≤ 0.05).