Table 2.

Dynamic light scattering (droplet size and polydispersity index) and zeta potential values after preparation (day 0) and after storage of the nano-emulsions prepared with the essential oil of Pectis elongata.

Nano-Emulsion (Replicate 1)
	Day 0	Day 1	Day 7	Day 14	Day 30	Day 60	Day 90
Size (nm)	119.8 ± 0.4509	117.4 ± 0.7638	114.2 ± 1.012	117.5 ± 0.4163	138.9 ± 0.1732	180.4 ± 1.286	217.7 ± 1.900
Polydispersity index	0.217 ± 0.018	0.221 ± 0.005	0.201 ± 0.009	0.158 ± 0.006	0.119 ± 0.013	0.086 ± 0.012	0.083 ±
Zeta potential	−3.66 ± 0.0902	−2.17 ± 0.148	−2.53 ± 0.127	−1.61 ± 0.142	−1.31 ± 0.0702	−8.05 ± 0.230	−0.279 ± 0.406
Nano-Emulsion (Replicate 2)
	Day 0	Day 1	Day 7	Day 14	Day 30	Day 60	Day 90
Size (nm)	113.4 ± 1.015	111.7 ± 0.6658	109.0 ± 0.3786	111.0 ± 0.8386	125.0 ± 0.4041	155.4 ± 1.834	180.5 ± 1.604
Polydispersity index	0.223 ± 0.018	0.209 ± 0.011	0.193 ± 0.006	0.171 ± 0.005	0.119 ± 0.016	0.091 ± 0.007	0.089 ± 0.026
Zeta potential	−2.34 ± 0.366	−1.44 ± 0.369	−3.04 ± 0.215	−1.77 ± 0.0757	−1.63 ± 0.429	−3.11 ± 0.527	−0.0173 ± 0.266
Nano-Emulsion (Replicate 3)
	Day 0	Day 1	Day 7	Day 14	Day 30	Day 60	Day 90
Size (nm)	115.8 ± 0.1155	114.2 ± 1.350	114.7 ± 0.1155	111.3 ± 0.1528	123.1 ± 1.015	155.0 ± 1.082	183.4 ± 1.058
Polydispersity index	0.204 ± 0.011	0.223 ± 0.017	0.226 ± 0.018	0.176 ± 0.008	0.120 ± 0.014	0.087 ± 0.022	0.074 ± 0.015
Zeta potential	−2.38 ± 0.123	−2.03 ± 0.0529	−4.40 ± 0.0896	−2.42 ± 0.199	−2.36 ± 0.0252	−3.42 ± 0.173	−1.69 ± 0.383
Average
	Day 0	Day 1	Day 7	Day 14	Day 30	Day 60	Day 90
Size (nm)	116.4 ± 2.868	114.4 ± 2.612	112.6 ± 2.820	113.3 ± 3.180	129.0 ± 7.484	163.6 ± 12.64	193.9 ± 19.96
Polydispersity index	0.215 ± 0.016	0.218 ± 0.012	0.207 ± 0.018	0.168 ± 0.010	0.119 ± 0.012	0.088 ± 0.013	0.082 ± 0.018
Zeta potential	−2.80 ± 0.681	−1.88 ± 0.390	−3.32 ± 0.847	−1.93 ± 0.391	−1.77 ± 0.515	−4.86 ± 2.41	−0.662 ± 0.838